The radical scavenging DPPH • method allows for a reaction with almost any type of antioxidant due to the stability of DPPH •. The method is based on the spectrophotometric measurement of the DPPH concentration change resulting from the reaction with an antioxidant. The final molar ratio of the radical and test compound was 10:1. These compounds have been described as chain-breaking antioxidants acting through radical scavenging activity, that is related to their hydrogen or electron donating capacity and to the ability to delocalize/stabilize the resulting phenoxyl radical within their structure. Using DPPH Radical scavenging assay to measure antioxidants in vegetable oil. With the aid of this reaction, a kinetic approach was adopted to assess the hydroxyl radical scavenging properties of polyphenolics, flavonoids and other compounds (e.g., ascorbic acid, glucose, mannitol). Several p This means there is sufficient time for even weak antioxidants to react with DPPH • . The 2,2-diphenylpicrylhydrazyl (DPPH) assay is widely used in plant biochemistry to evaluate the properties of plant constituents for scavenging free radicals. A second-order rate constant for the reaction of the scavenger with OH could be deduced from the inhibition of colour formation due to the salicylate probe. Sanchez-Moreno et al. Thus, the EPR-DPPH assay eliminated interference from the pigments coexisting in the reaction medium and improved the accuracy of the DPPH radical scavenging potential of such extracts. DPPH is an organic compound that is composed of stable free radical molecules and presents as a dark purple powder which turns clear when reduced by an antioxidant. DPPH + (H - A) → DPPH - H + A (Purple) (Yellow) The hydrogen atom- or electron-donation ability of the corresponding extracts and some pure compounds were This method can be used with both polar and nonpolar organic solvents to evaluate hydrophilic and lipophilic antioxidants . DPPH is a common abbreviation for the organic chemical compound 2,2-diphenyl-1-picrylhydrazyl. mol/L methanolic solution of DPPH. The ABTS•+ and DPPH assays are widely used methods for the assessment of the antioxidant capacities of natural products, they both are spectrophotometric techniques based on quenching of stable colored radicals (ABTS•+ or DPPH) and show the radical scavenging ability of antioxidants even when present in complex biological mixtures such as plant or food extracts. DPPH radical scavenging activity was measured as described previ-ously.4,5) To a solution of the DPPH radical (500mM, 1ml) in methanol or acetone was added a test solution (12.5mM, 4ml) in the same solvent in a test tube.

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